Jimmy Kimmel has said it was "never my intention to make light of" Charlie Kirk's death.
The presenter appeared almost tearful as he opened his first show since being taken off-air last week over comments about the assassination of the conservative influencer.
"I don't think there's anything funny about it," he said as he choked up.
"Nor was it my intention to blame any specific group for the actions of what was obviously a deeply disturbed individual. That was really the opposite of the point I was trying to make".
Kimmel was accused of being "offensive and insensitive" after using his programme, Jimmy Kimmel Live, to accuse Donald Trump and his allies of capitalising on the killing.
Speaking on Tuesday night's show, Kimmel said he understood why the remarks "felt either ill-timed or unclear, or maybe both".
'Not legal' to take me off-air, says Kimmel
Appearing tearful again, Kimmel praised Kirk's widow, Erika, for forgiving her husband's killer at his memorial service, calling it a "selfless act of grace ... that touched me deeply". In contrast, Mr Trump has said he disagreed, telling the service: "I hate my opponent and I don't want the best for them."
Kimmel went on to criticise the ABC affiliates who took his show off the air, saying: "That's not legal. That's not American. It's un-American."
He also thanked those who supported him, adding: "It takes courage for them to speak out against this administration. They did and they deserve credit for it."
Disney announced the return of the show to its ABC network after backlash to his suspension, although two major operators said they still wouldn't show it.
Read more from Sky News:
Trump backs Ukraine to retake all lost territory
Man found guilty of trying to assassinate US president
Trump threatens further action
In a post on his Truth Social platform before it aired on Tuesday night, Mr Trump said he "can't believe" ABC gave Kimmel his show back and hinted at further action.
"Why would they want someone back who does so poorly, who's not funny, and who puts the Network in jeopardy by playing 99% positive Democrat GARBAGE," he wrote.
"He is yet another arm of the DNC and, to the best of my knowledge, that would be a major illegal Campaign Contribution. I think we're going to test ABC out on this."
In his opening monologue, Kimmel mocked Mr Trump for criticising him for bad ratings, saying: "He tried his best to cancel me and instead he forced millions of people to watch this show."
Mr Trump had welcomed Kimmel's suspension, saying he had "bad ratings", but the move was criticised by Hollywood stars and senior Democrats including Barack Obama.
What did Kimmel say about Charlie Kirk?
The comments that saw Kimmel taken off-air were made last week.
The TV presenter said: "We hit some new lows over the weekend with the MAGA gang desperately trying to characterise this kid who murdered Charlie Kirk as anything other than one of them and doing everything they can to score political points from it."
Speaking about Mr Trump, he added: "This is not how an adult grieves the murder of someone he calls a friend.
"This is how a four-year-old mourns a goldfish."
"Many in MAGA land are working very hard to capitalise on the murder of Charlie Kirk," he continued.
This breaking news story is being updated and more details will be published shortly.
Please refresh the page for the fullest version.
You can receive Breaking News alerts on a smartphone or tablet via the Sky News App. You can also follow @SkyNews on X or subscribe to our YouTube channel to keep up with the latest news.
(c) Sky News 2025: Jimmy Kimmel addresses Charlie Kirk comments in first show since being taken off-air